Subject    :    [2017 Oct;41(5)] Generation of Insulin-Expressing Cells in Mouse Small Intestine by Pdx1, MafA, and BETA2/NeuroD
Writer KDA
Date 2018-12-03 18:30:06 Hit 1,888
Diabetes Metab J. 2017 Oct;41(5):405-416. English.
Published online Sep 05, 2017.  https://doi.org/10.4093/dmj.2017.41.5.405 
Copyright © 2017 Korean Diabetes Association
   
Generation of Insulin-Expressing Cells in Mouse Small Intestine by Pdx1, MafA, and BETA2/NeuroD
So-Hyun Lee, Marie Rhee, Ji-Won Kim and Kun-Ho Yoon
Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea.

Corresponding author: Kun-Ho Yoon. Division of Endocrinology and Metabolism, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 06591, Korea. Email: yoonk@catholic.ac.kr 
 
Received March 13, 2017; Accepted June 03, 2017.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

 
Abstract

Background

To develop surrogate insulin-producing cells for diabetes therapy, adult stem cells have been identified in various tissues and studied for their conversion into β-cells. Pancreatic progenitor cells are derived from the endodermal epithelium and formed in a manner similar to gut progenitor cells. Here, we generated insulin-producing cells from the intestinal epithelial cells that induced many of the specific pancreatic transcription factors using adenoviral vectors carrying three genes: PMB (pancreatic and duodenal homeobox 1 [Pdx1], V-maf musculoaponeurotic fibrosarcoma oncogene homolog A [MafA], and BETA2/NeuroD).

Methods

By direct injection into the intestine through the cranial mesenteric artery, adenoviruses (Ad) were successfully delivered to the entire intestine. After virus injection, we could confirm that the small intestine of the mouse was appropriately infected with the Ad-Pdx1 and triple Ad-PMB.

Results

Four weeks after the injection, insulin mRNA was expressed in the small intestine, and the insulin gene expression was induced in Ad-Pdx1 and Ad-PMB compared to control Ad-green fluorescent protein. In addition, the conversion of intestinal cells into insulin-expressing cells was detected in parts of the crypts and villi located in the small intestine.

Conclusion

These data indicated that PMB facilitate the differentiation of mouse intestinal cells into insulin-expressing cells. In conclusion, the small intestine is an accessible and abundant source of surrogate insulin-producing cells.

   
Keywords:
AdenoviridaeBeta cellsDifferentiationInjectionsIntestine, smallTranscription factors

Articles - Reviews in DMJ
No. Subject Date Hit ↓
Notice : [2019 Apr;43(2)] Associations between Breastfeeding and Type 2 Diabetes Mellitus and Glycemic Control in Parous Women: A Nationwide, Population-Based Study [2019 Apr;43(2)] Associations between Breastfeeding and Type 2 Diabe... 2019-04-18 1,079
Notice : [2019 Apr;43(2)] Increased Nociceptive Responses in Streptozotocin-Induced Diabetic Rats and the Related Expression of Spinal NR2B Subunit of N-Methyl-D-Aspartate Receptors [2019 Apr;43(2)] Increased Nociceptive Responses in Streptozotocin-I... 2019-04-18 820
Notice : [2019 Apr;43(2)] The Risk of Myocardial Infarction and Ischemic Stroke According to Waist Circumference in 21,749,261 Korean Adults: A Nationwide Population-Based Study [2019 Apr;43(2)] The Risk of Myocardial Infarction and Ischemic Stro... 2019-04-18 832
Notice : [2019 Apr;43(2)] Myricetin Protects Against High Glucose-Induced β-Cell Apoptosis by Attenuating Endoplasmic Reticulum Stress via Inactivation of Cyclin-Dependent Kinase 5 [2019 Apr;43(2)] Myricetin Protects Against High Glucose-Induced ... 2019-04-18 796
Notice : [2019 Apr;43(2)] Association between Changes in Anthropometric Indices and in Fasting Insulin Levels among Healthy Korean Adolescents: The JS High School Study [2019 Apr;43(2)] Association between Changes in Anthropometric Indic... 2019-04-18 803
Notice : [2019 Apr;43(2)] Discrepancies between Glycosylated Hemoglobin and Fasting Plasma Glucose for Diagnosing Impaired Fasting Glucose and Diabetes Mellitus in Korean Youth and Young Adults [2019 Apr;43(2)] Discrepancies between Glycosylated Hemoglobin and F... 2019-04-18 827
Notice : [2019 Apr;43(2)] Predictors of the Therapeutic Efficacy and Consideration of the Best Combination Therapy of Sodium-Glucose Co-transporter 2 Inhibitors [2019 Apr;43(2)] Predictors of the Therapeutic Efficacy and Consider... 2019-04-18 797
Notice : [2019 Apr;43(2)] Metabolic Consequences of Obstructive Sleep Apnea Especially Pertaining to Diabetes Mellitus and Insulin Sensitivity [2019 Apr;43(2)] Metabolic Consequences of Obstructive Sleep Apnea E... 2019-04-18 804
Notice : [2019 Apr;43(2)] Nonalcoholic Fatty Liver Disease and Diabetes: Part II: Treatment [2019 Apr;43(2)] Nonalcoholic Fatty Liver Disease and Diabetes: Part... 2019-04-18 808
283 [2019 Feb;43(1)] Higher High Density Lipoprotein 2 (HDL2) to Total H... 2019-03-06 157
282 [2019 Feb;43(1)] Proportion and Characteristics of the Subjects with... 2019-03-06 157
281 [2019 Feb;43(1)] Soluble Dipeptidyl Peptidase-4 Levels Are Associate... 2019-03-06 147
280 [2019 Feb;43(1)] Projection of Diabetes Prevalence in Korean Adults ... 2019-03-06 156
279 [2019 Feb;43(1)] High Proportion of Adult Cases and Prevalence of Me... 2019-03-06 89
278 [2019 Feb;43(1)] Association of Bisphenol A and Its Substitutes, Bis... 2019-03-06 106
277 [2019 Feb;43(1)] Hospital-Based Korean Diabetes Prevention Study: A ... 2019-03-06 112
276 [2019 Feb;43(1)] Nonalcoholic Fatty Liver Disease in Diabetes. Part ... 2019-03-06 129
275 [2019 Feb;43(1)] Update on the Impact, Diagnosis and Management of C... 2019-03-06 119
274 [2018 Dec;42(6)] The Prevalence and Risk Factors for Diabetic Retino... 2019-03-06 109

목록

Gets the previous 5 pages.   Go to previous page.  [1]   2   3   4   5     Go to next page. Gets the next  5 pages.