Subject    :    [2016 Dec;40(6)] Investigating Susceptibility to Diabetes Using Features of the Adipose Tissue in Response to In Utero Polycyclic Aromatic Hydrocarbons Exposure
Writer KDA
Date 2017-01-02 14:25:53 Hit 6,990
Diabetes Metab J. 2016 Dec;40(6):494-508. English.
Published online Aug 12, 2016.  https://doi.org/10.4093/dmj.2016.40.6.494 
Copyright © 2016 Korean Diabetes Association
   
Investigating Susceptibility to Diabetes Using Features of the Adipose Tissue in Response to In Utero Polycyclic Aromatic Hydrocarbons Exposure
Worlanyo E. Gato,1 Daniel A. Hunter,1 Shamaya L. Whitby,1 Christopher A. Mays,1 and Wilson Yau2
1Department of Chemistry, Georgia Southern University, Statesboro, GA, USA.
2Department of Pathology, University of Georgia College of Veterinary Medicine, Athens, GA, USA.

Corresponding author: Worlanyo E. Gato. Department of Chemistry, Georgia Southern University, 1332 Southern Dr, Statesboro, GA 30458, USA. Email: wgato@georgiasouthern.edu 
 
Received March 03, 2016; Accepted July 13, 2016.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.



Abstract

Background

In recent times, there has been an increase in the incidence of type 2 diabetes mellitus (T2DM) particularly in children. Adipocyte dysfunction provide a critical link between obesity and insulin resistance resulting in diabetes outcome. Further, environmental chemical exposure during early years of life might be a significant contributing factor to the increase in the incidence of T2DM. This study tests the idea that exposure to environmental contaminants (2-aminoanthracene [2AA]) in utero will show effects in the adipose tissue (AT) that signify T2DM vulnerability. 2AA is a polycyclic aromatic hydrocarbon found in a variety of products.

Methods

To accomplish the study objective, pregnant dams were fed various amounts of 2AA adulterated diets from gestation through postnatal period. The neonates and older offspring were analyzed for diabetic-like genes in the ATs and analysis of serum glucose. Furthermore, weight monitoring, histopathology and immunohistochemical (IHC) staining for CD68 in AT, adipocyte size determination and adiponectin amounts in serum were undertaken.

Results

Up-regulation of adiponectin and interleukin-6 genes were noted in the pups and older rats. Combination of intrauterine 2AA toxicity with moderate high fat diet exhibited gene expression patterns similar to those of the neonates. Elevated serum glucose levels were noted in treated groups. IHC of the AT indicated no significant malformations; however, CD68+ cells were greater in the animals treated to 2AA. Similarly, mean sizes of the adipocytes were larger in treated and combined 2AA and moderate high fat animals. Adiponectin was reduced in 2AA groups.

Conclusion

From the preceding, it appears intrauterine 2AA disturbance, when combined with excess fat accumulation will lead to greater risk for the diabetic condition.

   
Keywords:
Adipocytes; Adiponectin; Diabetes; Gene expression; Interleukin-6; Serum glucose; 2-Anthramine

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