Subject    :    [2019 Apr;43(2)] Increased Nociceptive Responses in Streptozotocin-Induced Diabetic Rats and the Related Expression of Spinal NR2B Subunit of N-Methyl-D-Aspartate Receptors
Writer KDA
Date 2019-04-18 10:34:02 Hit 451
LINK URL https://e-dmj.org/search.php?where=aview&id=10.4093/dmj.2018.0020&code=2004DMJ&vmode=FULL (Click 10)
Diabetes Metab J. 2019 Apr;43(2):222-235. English.
Published online Nov 19, 2018.  https://doi.org/10.4093/dmj.2018.0020 
Copyright © 2019 Korean Diabetes Association
   
Increased Nociceptive Responses in Streptozotocin-Induced Diabetic Rats and the Related Expression of Spinal NR2B Subunit of N-Methyl-D-Aspartate Receptors
Che Aishah Nazariah Ismail,1,2 Rapeah Suppian,1 Che Badariah Abd Aziz,2 Khalilah Haris,1 and Idris Long1
1School of Health Sciences, Universiti Sains Malaysia Health Campus, Kota Bharu, Malaysia.
2Physiology Department, School of Medical Sciences, Universiti Sains Malaysia Health Campus, Kota Bharu, Malaysia.

Corresponding author: Idris Long. School of Health Sciences, Universiti Sains Malaysia Health Campus, 16150 Kubang Kerian Kota Bharu, Kelantan, Malaysia. Email: idriskk@usm.my 
 
Received January 28, 2018; Accepted August 03, 2018.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

 
Abstract

Background

This study investigated the role of NR2B in a modulated pain process in the painful diabetic neuropathy (PDN) rat using various pain stimuli.

Methods

Thirty-two Sprague-Dawley male rats were randomly allocated into four groups (n=8): control, diabetes mellitus (DM) rats and diabetic rats treated with ifenprodil at a lower dose (0.5 µg/day) (I 0.5) or higher dose (1.0 µg/day) (I 1.0). DM was induced by a single injection of streptozotocin at 60 mg/kg on day 0 of experimentation. Diabetic status was assessed on day 3 of the experimentation. The responses on both tactile and thermal stimuli were assessed on day 0 (baseline), day 14 (pre-intervention), and day 22 (post-intervention). Ifenprodil was given intrathecally for 7 days from day 15 until day 21. On day 23, 5% formalin was injected into the rats' hind paw and the nociceptive responses were recorded for 1 hour. The rats were sacrificed 72 hours post-formalin injection and an analysis of the spinal NR2B expression was performed.

Results

DM rats showed a significant reduction in pain threshold in response to the tactile and thermal stimuli and higher nociceptive response during the formalin test accompanied by the higher expression of phosphorylated spinal NR2B in both sides of the spinal cord. Ifenprodil treatment for both doses showed anti-allodynic and anti-nociceptive effects with lower expression of phosphorylated and total spinal NR2B.

Conclusion

We suggest that the pain process in the streptozotocin-induced diabetic rat that has been modulated is associated with the higher phosphorylation of the spinal NR2B expression in the development of PDN, which is similar to other models of neuropathic rats.

   
Keywords:
Diabetic neuropathiesHyperalgesiaIfenprodilNR2B NMDA receptorPain measurement
 

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