Subject    :    [2019 Apr;43(2)] Increased Nociceptive Responses in Streptozotocin-Induced Diabetic Rats and the Related Expression of Spinal NR2B Subunit of N-Methyl-D-Aspartate Receptors
Writer KDA
Date 2019-04-18 10:34:02 Hit 873
LINK URL https://e-dmj.org/search.php?where=aview&id=10.4093/dmj.2018.0020&code=2004DMJ&vmode=FULL (Click 21)
Diabetes Metab J. 2019 Apr;43(2):222-235. English.
Published online Nov 19, 2018.  https://doi.org/10.4093/dmj.2018.0020 
Copyright © 2019 Korean Diabetes Association
   
Increased Nociceptive Responses in Streptozotocin-Induced Diabetic Rats and the Related Expression of Spinal NR2B Subunit of N-Methyl-D-Aspartate Receptors
Che Aishah Nazariah Ismail,1,2 Rapeah Suppian,1 Che Badariah Abd Aziz,2 Khalilah Haris,1 and Idris Long1
1School of Health Sciences, Universiti Sains Malaysia Health Campus, Kota Bharu, Malaysia.
2Physiology Department, School of Medical Sciences, Universiti Sains Malaysia Health Campus, Kota Bharu, Malaysia.

Corresponding author: Idris Long. School of Health Sciences, Universiti Sains Malaysia Health Campus, 16150 Kubang Kerian Kota Bharu, Kelantan, Malaysia. Email: idriskk@usm.my 
 
Received January 28, 2018; Accepted August 03, 2018.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

 
Abstract

Background

This study investigated the role of NR2B in a modulated pain process in the painful diabetic neuropathy (PDN) rat using various pain stimuli.

Methods

Thirty-two Sprague-Dawley male rats were randomly allocated into four groups (n=8): control, diabetes mellitus (DM) rats and diabetic rats treated with ifenprodil at a lower dose (0.5 µg/day) (I 0.5) or higher dose (1.0 µg/day) (I 1.0). DM was induced by a single injection of streptozotocin at 60 mg/kg on day 0 of experimentation. Diabetic status was assessed on day 3 of the experimentation. The responses on both tactile and thermal stimuli were assessed on day 0 (baseline), day 14 (pre-intervention), and day 22 (post-intervention). Ifenprodil was given intrathecally for 7 days from day 15 until day 21. On day 23, 5% formalin was injected into the rats' hind paw and the nociceptive responses were recorded for 1 hour. The rats were sacrificed 72 hours post-formalin injection and an analysis of the spinal NR2B expression was performed.

Results

DM rats showed a significant reduction in pain threshold in response to the tactile and thermal stimuli and higher nociceptive response during the formalin test accompanied by the higher expression of phosphorylated spinal NR2B in both sides of the spinal cord. Ifenprodil treatment for both doses showed anti-allodynic and anti-nociceptive effects with lower expression of phosphorylated and total spinal NR2B.

Conclusion

We suggest that the pain process in the streptozotocin-induced diabetic rat that has been modulated is associated with the higher phosphorylation of the spinal NR2B expression in the development of PDN, which is similar to other models of neuropathic rats.

   
Keywords:
Diabetic neuropathiesHyperalgesiaIfenprodilNR2B NMDA receptorPain measurement
 

Articles - Reviews in DMJ
No. Subject Date Hit ↑
Notice : [2019 June;43(3)] Mitochondrial Dysfunction in Adipocytes as a Primary Cause of Adipose Tissue Inflammation [2019 June;43(3)] Mitochondrial Dysfunction in Adipocytes as a Prima... 2019-06-18 58
Notice : [2019 Apr;43(2)] Associations between Breastfeeding and Type 2 Diabetes Mellitus and Glycemic Control in Parous Women: A Nationwide, Population-Based Study [2019 Apr;43(2)] Associations between Breastfeeding and Type 2 Diabe... 2019-04-18 1,137
Notice : [2019 Apr;43(2)] Increased Nociceptive Responses in Streptozotocin-Induced Diabetic Rats and the Related Expression of Spinal NR2B Subunit of N-Methyl-D-Aspartate Receptors [2019 Apr;43(2)] Increased Nociceptive Responses in Streptozotocin-I... 2019-04-18 873
Notice : [2019 Apr;43(2)] The Risk of Myocardial Infarction and Ischemic Stroke According to Waist Circumference in 21,749,261 Korean Adults: A Nationwide Population-Based Study [2019 Apr;43(2)] The Risk of Myocardial Infarction and Ischemic Stro... 2019-04-18 890
Notice : [2019 Apr;43(2)] Myricetin Protects Against High Glucose-Induced β-Cell Apoptosis by Attenuating Endoplasmic Reticulum Stress via Inactivation of Cyclin-Dependent Kinase 5 [2019 Apr;43(2)] Myricetin Protects Against High Glucose-Induced ... 2019-04-18 840
Notice : [2019 Apr;43(2)] Association between Changes in Anthropometric Indices and in Fasting Insulin Levels among Healthy Korean Adolescents: The JS High School Study [2019 Apr;43(2)] Association between Changes in Anthropometric Indic... 2019-04-18 853
Notice : [2019 Apr;43(2)] Discrepancies between Glycosylated Hemoglobin and Fasting Plasma Glucose for Diagnosing Impaired Fasting Glucose and Diabetes Mellitus in Korean Youth and Young Adults [2019 Apr;43(2)] Discrepancies between Glycosylated Hemoglobin and F... 2019-04-18 877
Notice : [2019 Apr;43(2)] Predictors of the Therapeutic Efficacy and Consideration of the Best Combination Therapy of Sodium-Glucose Co-transporter 2 Inhibitors [2019 Apr;43(2)] Predictors of the Therapeutic Efficacy and Consider... 2019-04-18 846
Notice : [2019 Apr;43(2)] Metabolic Consequences of Obstructive Sleep Apnea Especially Pertaining to Diabetes Mellitus and Insulin Sensitivity [2019 Apr;43(2)] Metabolic Consequences of Obstructive Sleep Apnea E... 2019-04-18 853
Notice : [2019 Apr;43(2)] Nonalcoholic Fatty Liver Disease and Diabetes: Part II: Treatment [2019 Apr;43(2)] Nonalcoholic Fatty Liver Disease and Diabetes: Part... 2019-04-18 857
283 [2017 Jun;41(3)] The Effect of 12 Weeks Aerobic, Resistance, and Com... 2017-07-06 9,021
282 [2017 Jun;41(3)] Satisfaction Survey on Information Technology-Based... 2017-07-06 8,939
281 [2017 Jun;41(3)] Impact of Gender on the Association between Low Ser... 2017-07-06 8,719
280 [2017 Jun;41(3)] Comparison of Antidiabetic Regimens in Patients wit... 2017-07-06 8,524
279 [2017 Jun;41(3)] Physician-Directed Diabetes Education without a Med... 2017-07-06 8,420
278 [2017 Apr;41(2)] Association between Blood Mercury Level and Viscera... 2017-07-06 8,390
277 [2017 Apr;41(2)] Addition of Ipragliflozin to Metformin Treatment in... 2017-07-06 8,371
276 [2017 Apr;41(2)] Diabetes Camp as Continuing Education for Diabetes ... 2017-07-06 8,343
275 [2017 Apr;41(2)] Role of NO/VASP Signaling Pathway against Obesity-R... 2017-07-06 8,332
274 [2017 Jun;41(3)] Diabetes Prevention in Australia: 10 Years Results ... 2017-07-06 8,301

목록

Gets the previous 5 pages.   Go to previous page.  [1]   2   3   4   5     Go to next page. Gets the next  5 pages.